Evolution of the NUDX1 gene in wild roses
Baptiste Nairaud, Sun Pulu, Aurélie Bony, Saretta Paramita, Crentin Conart, Sylvie Baudino, Jean-Claude Caissard  1@  
1 : Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales  (BVPAM)  -  Website
CNRS : FRE3727, Université Jean Monnet - Saint-Etienne : EA3061
Université Jean Monnet, 23 rue du Dr Michelon, F-42023 StEtienne cedex -  France

Previously, a new enzyme involved in geraniol biosynthesis has been characterised in modern roses. This enzyme, named NUDX1, does not belong to terpene synthases but to Nudix hydrolases (Magnard et al. 2015 Science 349, 81-83). In other organisms like Escherichia coli, Arabidopsis thaliana and Homo sapiens, NUDX1 homologs are involved in cellular sanitization, hydrolysing 8-oxo-dGTP to avoid mutations. In modern roses, it hydrolyses geranyl diphosphate into geranyl monophosphate. This compound is then probably hydrolysed into geraniol by an unknown enzyme. To gain knowledge about this new function of NUDX1 and its evolution, we have begun the characterisation of NUDX1 gene orthologs in wild species of roses and in other Rosaceae. We would like to know whether this function has appeared during domestication (30,000 cultivars created by growers since the XVIIth century), during evolution of the genus Rosa (120 species all around the world), or during the evolution of Rosaceae and closed families.


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